{"id":31686,"date":"2013-06-07T06:39:32","date_gmt":"2013-06-07T11:09:32","guid":{"rendered":"http:\/\/piel-l.org\/blog\/?p=31686"},"modified":"2013-06-08T06:51:09","modified_gmt":"2013-06-08T11:21:09","slug":"apoptotic-induction-of-skin-cancer-cell-death-by-plant-extracts","status":"publish","type":"post","link":"https:\/\/piel-l.org\/blog\/31686","title":{"rendered":"Apoptotic induction of skin cancer cell death by plant extracts."},"content":{"rendered":"<p><em>Thuncharoen W, Chulasiri M, Nilwarangkoon S, Nakamura Y, Watanapokasin R.<\/em><\/p>\n<p><!--more--><\/p>\n<p><strong>Source<\/strong><\/p>\n<p>Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.<\/p>\n<p><strong>Abstract<\/strong><\/p>\n<p><strong>OBJECTIVE<\/strong>:<\/p>\n<p>The aim of the present study was to investigate the effects of plant extracts on cancer apoptotic induction.<\/p>\n<p><strong>MATERIAL AND METHOD<\/strong>:<\/p>\n<p>Human epidermoid carcinoma A431 cell line, obtained from the American Type Culture Collection (ATCC, Manassas, VA), was maintained in Dulbecco&#8217;s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 degrees C, 5% carbon dioxide (CO2). Plant extract solutions were obtained from S &amp; J international enterprises public company limited. These plant extracts include 50% hydroglycol extracts from Etlingera elatior (Jack) R.M.Smith (torch ginger; EE), Rosa damascene (damask rose; DR) and Rafflesia kerrii Meijer (bua phut; RM). The cell viability, time and dose dependency were determined by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. A431 cells were treated with the plant extracts and stained with Hoechst 33342 fluorescent staining dye.<\/p>\n<p><strong>RESULTS<\/strong>:<\/p>\n<p>Cell viability was demonstrated by the inhibitory concentration 50% (IC50). The anti-proliferative effects were shown to be dependent on time and dose. Typical characteristics of apoptosis which are cell morphological changes and chromatin condensation were clearly observed.<\/p>\n<p><strong>CONCLUSION<\/strong>:<\/p>\n<p>The plant extracts was shown to be effective for anti-proliferation and induction of apoptosis cell death in skin cancer cells. Therefore, mechanisms underlying the cell death and its potential use for treatment of skin cancer will be further studied.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Thuncharoen W, Chulasiri M, Nilwarangkoon S, Nakamura Y, Watanapokasin R.<\/p>\n","protected":false},"author":16,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8,1],"tags":[],"class_list":["post-31686","post","type-post","status-publish","format-standard","","category-articulos-cientificos","category-generales"],"_links":{"self":[{"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/posts\/31686","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/comments?post=31686"}],"version-history":[{"count":0,"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/posts\/31686\/revisions"}],"wp:attachment":[{"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/media?parent=31686"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/categories?post=31686"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/piel-l.org\/blog\/wp-json\/wp\/v2\/tags?post=31686"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}